Am J Clin Exp Immunol 2013;2(2):186-194
DNA structures decorated with cathepsin G/secretory leukocyte proteinase
inhibitor stimulate IFNI production by plasmacytoid dendritic cells
Joanna Skrzeczynska-Moncznik, Agnieszka Wlodarczyk, Magdalena Banas, Mateusz Kwitniewski, Katarzyna Zabieglo,
Monika Kapinska-Mrowiecka, Adam Dubin, Joanna Cichy
Department of Immunology, Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and
Biotechnology, Jagiellonian University, Kraków, Poland; Department of Dermatology, Zeromski Hospital, Kraków, Poland.
These authors contributed equally to this work.
Received March 15, 2013; Accepted May 16, 2013; Epub June 15, 2013; Published June 30, 2013
Abstract: Plasmacytoid dendritic cells (pDCs) and neutrophils are detected in psoriatic skin lesions and implicated in
the pathogenesis of psoriasis. pDCs specialize in the production of type I interferon (IFNI), a cytokine that plays an
important role in chronic autoimmune-like inflammation, including psoriasis. Here, we demonstrate that IFNI production
in pDCs is stimulated by DNA structures containing the neutrophil serine protease cathepsin G (CatG) and the secretory
leukocyte protease inhibitor (SLPI), which is a controlling inhibitor of serine proteases. We also demonstrate the
presence of neutrophil-derived DNA structures containing CatG and SLPI in lesional skin samples from psoriasis
patients. These findings suggest a previously unappreciated role for CatG in psoriasis by linking CatG and its inhibitor
SLPI to the IFNI-dependent regulation of immune responses by pDCs in psoriatic skin. (AJCEI1303005).
Keywords: Serine protease, neutrophil extracellular traps, psoriasis, autoimmunity
Address correspondence to: Dr. Joanna Cichy, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian
University, Gronostajowa 7 St., 30-387 Krakow, Poland. Phone: 48-12-664 6127; Fax: 48-12-664 6904; E-mail:
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